ABSTRACT This work evaluated the productive and structural characteristics of the BRS Zuri and BRS Quênia cultivars under rotational stocking management. The evaluations were divided into five periods according to rainfall regime (high, medium, and low rainfall) during 16 months of the years 2016-2018. Pastures were grazed when canopies intercepted 95% of incident light to stubbles corresponding to 50% of the pre-grazing height. The pre- and post-grazing heights varied as a function of cultivar, with higher values for BRS Zuri (89 and 49 cm) than for BRS Quênia (78 and 45 cm). No differences in canopy lowering percentage, forage mass, and stem and dead material dry masses between cultivars were observed, with averages of 41.3%, 5,856, 1,835, and 841 kg ha−1, respectively. The post-grazing canopy height was lower in the second year, which resulted in higher values of canopy lowering percentage (51%). The highest value for leaf:stem ratio for both cultivars was observed in the high rainfall period of the second year. Higher average tiller population density was observed for BRS Quênia (477 tillers m−2) than for BRS Zuri (260 tillers m−2) for all the rainfall periods. Forage bulk density was similar between cultivars and varied according to the periods evaluated, with the highest value observed during the dry period of the year. Forage mass, as well as its component fractions, did not vary between cultivars, but was influenced by the studied periods. The cultivars showed high leaf mass in the pre-grazing forage mass with an average value of 3,174 kg ha−1. The cultivars are similar in morphological composition and offer high yield potential when managed under a rotational grazing system.

ABSTRACT. This study aimed to evaluate the shading influence on production and qualitative traits of four tropical forage legumes (calopo, puero, archer and perennial soybean). Legumes were cultivated under levels of artificial shading (0; 30; 50 and 70%) from January 2006 to June 2007. Dry matter production (DM), crude protein (CP), neutral detergent fiber (NDF), cellulose (CEL), hemicellulose (HEM) and lignin contents were evaluated. In the summer, increases in legumes DM yield were observed when grown under increasing shading levels. Calopo, puero, and archer production showed quadratic effect. Regression analysis derivation for dry matter yield showed levels of maximum values at 35.0; 33.78 and 45.45% in the summer and 34.94; 34.83 and 33.29% in the fall. Significant effect of species for CP, NDF, CEL, HEM and L/S (leaf/stem) ratio and shading level effect for NDF, CEL, HEM and L/S ratio were observed in the summer, however, no interaction was reported. In addition, significant effect of species for CP, NDF, CEL, HEM and L/S ratio was observed in the autumn. Only L/S ratio presented a negative response according to increasing shading levels. The shading promoted an increase in the fiber components and a decrease in the leaf/stem ratio, and the perennial soybean was the most tolerant to the increased shading.

ABSTRACT. The effect of ammonization was evaluated with urea at doses of 1, 2, 3 and 4%, on the basis of dry matter (DM) on Tifton 85 hay harvested at an advanced stage of development in the periods of 30 and 45 days, through analysis of dry matter, ash, ether extract (EE), neutral (NDF) and acid (ADF) detergent fiber, NDF corrected for ash and protein (NDFap), cellulose, lignin, crude protein (CP), acid detergent insoluble nitrogen (ADIN), fractionation of carbohydrates and in vitro gas production (Parameters: V f1 , k 1 , V f2 , k 2 and L). The variables DM, EE, NDF, ADF, lignin, cellulose, NDFap, fraction A + B1, fraction B2 and C of carbohydrates, and the V f1 and V f2 parameters were influenced by treatments (p < 0.05). While DM, NDF, NDFap, fraction A + B1, C, CP and V f2 showed linear effects according to the dose, NIDA and hemicellulose presented quadratic effects. V f1 and V f2 were also influenced by the treatment period. The ammonization with 2.7% urea on the basis of DM promotes improvement of Tifton 85 hay treated for 45 days, observed after derivation of the regression equation and evaluation of the nutritional and kinetic degradation in vitro.

RESUMO Objetivou-se avaliar características produtivas e qualitativas de Brachiaria decumbens e Brachiaria ruziziensis submetidas a três níveis de sombreamento artificial (0, 36 e 54%) e quatro doses de nitrogênio (N) (0, 50, 100 e 150 mg dm-3 de solo), utilizando-se o delineamento inteiramente casualizado, em esquema fatorial 2 x 3 x 4, com três repetições. Avaliou-se a produção de matéria seca (PMS), o número de perfilhos, a massa de raiz e os teores de proteína bruta (PB) e de fibra em detergente neutro (FDN). Ambas as gramíneas responderam quadraticamente ao N, mas a B. ruziziensis foi mais produtiva nas duas maiores doses de N. A densidade de perfilhos aumentou com a dose de N e diminuiu com o sombreamento. O peso de raiz aumentou com a dose de N, linearmente no sol pleno e quadraticamente na sombra. O sombreamento e o N influenciaram positivamente os teores de PB. Para os teores de FDN, houve redução com o aumento da dose de N. Para a B. decumbens, os teores de FDN aumentaram com o sombreamento; para B. ruziziensis o maior valor foi observado com sombreamento intermediário. A fertilização nitrogenada é uma importante estratégia para o aumento da PMS, do número de perfilhos e dos teores de PB da forragem, para ambas as gramíneas. Contudo, o sombreamento severo deve ser evitado, pois reduz o perfilhamento e o peso de raiz, o que pode comprometer a persistência da pastagem.

ABSTRACT This study aimed to evaluate the productive and qualitative characteristics of Brachiaria decumbens and Brachiaria ruziziensis subjected to three levels of artificial shading (0, 36 and 54%) and four nitrogen (N) doses (0; 50; 100 and 150 mg dm-3 soil), using completely randomized design with a factorial scheme 2 x 3 x 4, with three replications. The dry matter production (DMP), the number of tillers per pot, root weight and crude protein (CP) and neutral detergent fiber (NDF) contents were evaluated. Both grasses responded quadractly to N, but B. ruziziensis presented greater production under the two highest N doses. The tillers density increased with N dose and was reduced under shading. The root weight increased with N dose, linearly in the full sun and quadractly in the shade. The shading and N showed a positive influence on CP contents. For NDF content, was observed reduction with increase of N dose. The NDF content of B. decumbens increased with shading levels; for B. ruziziensis, the greatest value was observed under intermediate shading level. The N fertilization is an important strategy to improve DMP, tillers density and CP content, for both grasses. However, intense shading should be avoided, as it reduces tillering and root weight, which may threat pasture persistence.

Resumo: O objetivo deste trabalho foi avaliar o efeito da inclusão de soja (Glycine max), feijão-guandu (Cajanus cajan) e feijão-de-porco (Canavalia ensiformis) como fonte de urease para feno de capim-elefante (Pennisetum purpureum) amonizado com ureia. O delineamento experimental foi inteiramente casualizado, em arranjo fatorial duplo, com um tratamento adicional: 4 níveis de fonte de urease x 3 fontes de urease + 1 controle. Realizou-se a análise química-bromatológica e o fracionamento de carboidratos no feno, e a produção cumulativa de gás in vitro foi determinada. Houve efeito da interação entre o nível e a fonte de urease para fibra em detergente neutro e fibra em detergente ácido, em que a adição de 1 e 2% de feijão-de-porco resultou nos menores valores de fibra em detergente ácido, e a adição de 2% de feijão-de-porco e 3% de soja, nos menores valores de lignina. A adição de grãos moídos das leguminosas reduz os componentes da fibra e aumenta o teor de nitrogênio não proteico do feno de capim-elefante amonizado com ureia. A adição de 4% de grão de soja moído aumenta a produção de gás proveniente da fração solúvel.

Abstract: The objective of this work was to evaluate the use of soybean (Glycine max), pigeon pea (Cajanus cajan), and jack bean (Canavalia ensiformis) as urease sources for elephant grass (Pennisetum purpureum) hay ammoniated with urea. The experimental design was completely randomized in a double factorial arrangement with one additional treatment: 4 urease source levels x 3 urease sources + 1 control. Chemical-bromatological analyses and carbohydrate fractionation were performed in the hay, and cumulative gas production in vitro was determined. There were interactions between urease level and source for neutral detergent fiber and acid detergent fiber, in which 1 and 2% jack bean lowered acid detergent fiber values, and 2% jack bean and 3% soybean reduced lignin content. The addition of milled legume grains reduces fiber components and increases non protein nitrogen content in elephant grass hay ammoniated with urea. Adding 4% milled soybean increases gas production in the soluble fraction.

Resumo: O objetivo deste trabalho foi avaliar o perfil de ácidos graxos (AG) do leite de vacas Holandesas x Gir submetidos a dois tipos de manejo de pastejo (períodos de descanso fixo e variável) de pastagem de Urochloa brizantha 'Marandu'. Utilizou-se o delineamento de blocos ao acaso, com duas repetições de área de pastagem (blocos) por tratamento e quatro vacas por bloco. A produção e a composição do leite não foram influenciadas pelas estratégias de pastejo. Não houve efeito dos tratamentos sobre as proporções (g 100 g-1 de AG totais) dos principais AG (palmítico, linoleico e α-linolênico) do pasto, mas suas ingestões (gramas por dia) foram influenciadas pelas diferenças de ingestão de matéria seca da forragem. As concentrações dos AG no plasma e na gordura do leite não foram influenciadas pelos tratamentos. As concentrações dos ácidos rumênico, vacênico, oleico e α-linolênico na gordura do leite variaram de 0,71 a 0,93, 1,40 a 1,50, 19,40 a 19,70 e 0,39 a 0,43 g 100 g-1 de AG totais, respectivamente. As estratégias de pastejo de U.brizantha 'Marandu' não alteram o perfil de ácidos graxos do leite das vacas.

Abstract: The objective of this work was to evaluate the milk fatty acid (FA) profile of Holstein x Gyr cows subjected to two different grazing managements (fixed and variable rest periods) of Urochloa brizantha 'Marandu' pastures. A randomized complete block design was used, with two replicates of pasture areas (blocks) per treatment and four cows per block. Milk production and composition were not affected by grazing strategies. No treatment effects were observed on the proportions (g 100 g-1 of total FA) of the main FAs (palmitic, linoleic, and α-linolenic) of the pasture, but their intakes (grams per day) were affected by differences in forage dry matter intake. The concentrations of FAs in milk plasma and fat were not affected by the treatments. Milk fat contents of rumenic, vaccenic, oleic, and α-linolenic acids varied from 0.71 to 0.93, 1.40 to 1.50, 19.40 to 19.70, and 0.39 to 0.43 g 100 g-1 total FAs, respectively. Grazing strategies of U.brizantha 'Marandu' cause no changes on the milk fatty acid profile of cows.

RESUMO: Os sistemas silvipastoris têm sido sugeridos como alternativa para recuperação de pastagens em regiões tropicais. Entretanto, as árvores reduzem a disponibilidade de radiação para o pasto, o que pode influenciar no crescimento e acúmulo de forragem. O objetivo deste estudo foi avaliar a morfogênese, a estrutura do dossel e o acúmulo de forragem de Brachiaria decumbens , submetida a três regimes de luz (0, 20 e 70% de sombreamento natural) e dois níveis de fertilização (presença ou ausência de nitrogênio, fósforo e potássio). As taxas de alongamento de folhas e colmos aumentaram com o sombreamento, mas não variaram com a fertilização. A taxa de aparecimento de folhas foi maior com a fertilização, mas, em geral, foi semelhante entre os níveis de sombra. A densidade de perfilhos foi maior no sol pleno e menor na sombra intensa. A densidade de perfilhos respondeu à fertilização sob sol pleno e sombra moderada. O acúmulo de forragem aumentou 42% com a fertilização no sol pleno, 12% na sombra moderada e não variou na sombra intensa. Os resultados mostraram que, mesmo com uso de fertilização, o acúmulo de forragem foi limitado pela redução de luz. Em condições de sombra moderada, a fertilização foi importante para aumentar a população de perfilhos ao longo dos ciclos de crescimento.

ABSTRACT: The silvopastoral system has been suggested as an alternative to recover degraded pastures in tropical regions. However, trees reduce the light available for pastures, which may affect the growth and herbage accumulation. The objective of this study was to evaluate the morphogenesis, canopy structure and herbage accumulation of signalgrass ( Brachiaria decumbens ) subjected to three light regimes (0, 20 and 70% of natural shading) and two fertilization levels (presence or absence of nitrogen, phosphorus and potassium). Leaf and stem elongation rates increased under shading but did not vary with fertilization. The leaf appearance rate was greater under fertilizer treatment but was generally similar among light regimes. The tiller density was greater in full sun and lower in intense shading. Tiller density responded to fertilization under full sun and moderate shading. Herbage accumulation increased by 42% with fertilization under full sun, 12% under moderate shading and did not vary under intense shading. Results showed that even under fertilization the herbage accumulation was limited by reduced light. However, under moderate shade the fertilization was important to raise tiller population over the growth cycles.

RESUMO A avaliação do potencial produtivo do pasto não é o único fator que pode ser usado para determinar a eficiência de manejo do pastejo, também é necessário considerar as características estruturais do dossel. Neste estudo, objetivou-se avaliar a influência de dois critérios para definição do período de descanso do pasto de capim-marandu sobre a massa de forragem, a composição morfológica e as características estruturais do pasto durante a estação chuvosa. Os períodos de descanso estudados foram: fixo de 30 dias (PD30) e variável, conforme a interceptação de 95% da radiação fotossinteticamente ativa (IL95). O delineamento experimental utilizado foi em blocos inteiramente casualizados, com duas repetições de área e três repetições dentro de bloco. Os animais (vacas mestiças) foram colocados nos piquetes, logo que os pastos atingiam os critérios de manejo estabelecidos e permaneciam dentro dos mesmos durante três dias, de forma a atingir a altura de resíduo de 20-25 cm. Menor intervalo entre pastejo (22,8 vs. 30 dias), menor altura pré-pastejo (35,9 vs. 42,3 cm) e menor massa de forragem verde (4.411 vs. 5.290 kg/ha.ciclo) foram observados no pasto sob IL95. Contudo, o pasto sob IL95 apresentou maior percentual de lâmina foliar (48,3 vs. 41,1) e menor percentual de material morto (19,0 vs. 25,4) na massa de forragem pré-pastejo em relação ao pasto com PD30. O manejo do pasto de capim-marandu, baseado em período de descanso fixo de 30 dias, ao longo da estação chuvosa, compromete a estrutura do pasto, reduzindo a participação de folhas e aumentando os percentuais de colmo e material senescente na forragem disponível. A menor massa de forragem em pré-pastejo observada sob IL95 é compensada pelo maior número de ciclos de pastejo dentro da estação chuvosa.

ABSTRACT An evaluation of productive potential is not the only factor used to determine grazing management efficiency; it is also necessary to know the structural characteristics of the canopy. The objective of this study was to evaluate the effects of two management styles on Marandu palisade grass pastures under rotational stocking with respect to the herbage mass, morphological composition, and structural characteristics of the pasture. The treatments under consideration were a) a fixed rest period of 30 days (RP30) or b) a rest period based on the time necessary for the canopy to reach 95% light interception (LI95). The experimental design was a completely randomized block with two blocks and three replicates within the blocks. Animals were placed in the paddocks when the grass reached the established management criteria, which were maintained for three days to reach 25 cm in stubble height. Shorter grazing intervals (22.8 vs. 30 days), lower pre-grazing heights (35.9 vs. 42.3 cm), and lower forage masses (4,411 vs. 5,290 kg/ha.cycle) were observed in LI95 pastures. The LI95 treatment had a higher leaf percentage (48.3 vs. 41.1) and the lowest percentage of dead forage (19.0 vs. 25.4) in the pre-grazing forage mass of the pasture in relation to that of RP30. Performing pasture management based on RP30 throughout the season is harmful to the pasture structure because it reduces the leaf percentage and increases the stem and senescent material in the available forage. The lower pre-grazing forage mass observed under LI95 management is offset by more grazing cycles within the rainy season.

ABSTRACTThe objective of this work was to evaluate the inclusion of Fabaceae Flemingia macrophylla (Willd.) Kuntze ex Merr. in the diet of lactating dairy goats arranged in a 5 × 5 Latin square. The diets were composed of 40% of concentrate and 60% of roughage, and the dietary treatments were defined by the level of Flemingia hay inclusion (0%, 8%, 16%, 24%, and 32% in the diet dry matter) replacing Cynodon dactyloncv. Tifton 85 hay. The diets were isonitrogenous, with 14% crude protein. Feed intake, nutrient digestibility, feeding behavior, and ruminal pH and ammonia nitrogen were evaluated. There was no difference in dry matter intake with the inclusion of Flemingia hay in the diet. The digestibility of dry matter, organic matter, crude protein, neutral detergent fiber, and total carbohydrates decreased with the inclusion of Flemingia in the diet. The diet did not change rumen ammonia nitrogen concentration or ruminal pH. There were no differences in the feeding behavior or feed and rumination efficiencies. Flemingia macrophylla can be used up to the level of 32% in the dry matter in diets for lactating goats.

O objetivo do presente experimento foi avaliar as respostas de desempenho de caprinos castrados, submetidos a dois tratamentos da ferida cirúrgica: tratamento convencional com pomada à base de óxido de zinco, óleo de pinho, caulim e xilol e spray à base de permetrina, fenoxibenzil e DDVP (diclorvós) e tratamento alternativo com uso de fitoterápico com pomada à base de extrato de Calendula offcinalis e associações. Foram utilizados doze cabritos sem raça definida com peso inicial médio de 15,5 kg, criados em confinamento total, separados em dois grupos dispostos em delineamento inteiramente casualizado para constituir dois tratamentos com seis repetições cada, visando comparar o desempenho dos animais tratados de forma convencional em relação ao tratamento com extrato de Calendula offcinalis e associações. Empregada a técnica de orquiectomia (castração) por abertura da bolsa escrotal e com cordão espermático descoberto no dia 0, os dois grupos foram tratados até a completa cicatrização da ferida cirúrgica no 51º dia de experimentação. As medidas biométricas analisadas foram ganho de peso (kg), ganho de altura de cernelha (cm), ganho de altura de garupa (cm), ganho de perímetro torácico (cm) e escore de condição corporal. Os resultados foram analisados pelo pacote estatístico SAEG e teste de Tukey (P>0,05). Não houve diferença significativa entre os tratamentos (P>0,05) para nenhum dos parâmetros analisados. Todas as características biométricas, à exceção do escore corporal, aumentaram no período de avaliação. Não houve impacto negativo no uso de fitoterápico em relação às características observadas.

The purpose of this experiment was to evaluate the performance responses of castrated goats, submitted to two surgical wound treatments: conventional treatment with ointment made of zinc oxide, pine oil, kaolin and xylene and permethrin, phenoxybenzyl and DDVP spray (dichlorvos) and alternative phytotherapic treatment using herbal medicine with ointment made of Calendula offcinalis extract and associations. Twelve undefined breed goats with an average initial weight of 15.5 kg, raised in total confinement, were separated into two groups and arranged in a completely randomized design to constitute two treatments with six replicates each, in order to compare the performance of animals treated conventionally and with Calendula offcinalis extract and associations. We employed the orchiectomy (castration) technique by opening the scrotum and spermatic cord on day 0, and both groups were treated until complete healing of the surgical wound on the 51st day of trial. We analyzed the following biometric parameters: weight gain (kg), withers height gain (cm), croup height gain (cm), thotacic perimeter gain (cm) and body condition score. The results were analyzed using the Statistical Package SAEG and Tukey's test (P> .05). There was no significant difference between treatments (P> 0.05) for any of the parameters analyzed. All biometric characteristics, with the exception of body condition, increased during the evaluation period. There was no negative impact of the use of herbal medicine on the characteristics observed.

O propósito deste trabalho foi verificar o desempenho de um novo clorofilometro portátil, em parcelas experimentais vegetadas com o capim-Tifton 85 (Cynodon spp.). Para tal, foi utilizado um experimento de curta duração, em que três doses de N (0,75 e 150kg de N ha-1) foram combinadas com quatro datas de amostragem (8, 16, 24 e 28 dias após um corte de uniformização). As análises foram feitas em lâminas da folha mais nova totalmente expandida. Os teores das clorofilas a, b e a+b foram quantificados espectrofotometricamente após a extração com acetona 80%, enquanto que o clorofilômetro forneceu os correspondentes valores de Índice de Clorofila Falker (ICF). As leituras do aparelho foram sensíveis tanto às doses de N quanto às datas de amostragem. Foram obtidas correlações positivas entre ambos os métodos de quantificação (clorofila a: r=0,646; b: r=0,797; e total: r=0,721), que evidenciaram a utilidade desse novo clorofilometro na determinação indireta dos teores de clorofila foliar no capim-Tifton 85.

The purpose of this research was to evaluate the performance of a new portable chlorophyll meter in the indirect determination of chlorophyll contents in Tifton 85 bermudagrass leaves. In a field trial of short duration, three N doses (0, 75 and 150kg ha-1) were combined with four sampling dates (8, 16, 24 e 28 days after a cut for sward uniformity) in completely randomized blocks, with treatments arranged in a split-plot design. Analyses were performed in the youngest fully expanded leaf blade. Contents of chlorophylls a, b and total were quantified spectrophotometrically, after extraction with acetone 80%, while chlorophyll meter readings, expressed as Falker Chlorophyll Index (FCI) units, provided the same information. Chlorophyll meter readings were sensitive to both N rates and sampling dates. It were obtained significant positive correlations between both methods (chlorophyll a: r=0.646, chlorophyll b: r=0.797 and for total chlorophyll: r=0.721). It was concluded that this new chlorophyll meter constitutes an appropriate tool for the indirect determination of foliar chlorophyll contents in Tifton 85 pastures.

Production and quality of milk from dairy cows grazing exclusively on Mombasa grass pasture, during the spring-summer period and fed roughage supplementations, during the autumn-winter, were evaluated in two consecutive years. The pasture of 11.4 ha was divided into 57 paddocks of 2,000 m² each, including three replications with 19 paddocks each. The pasture was managed with two occupation days and 36 resting days. During the spring-summer period, cows received only grass pasture. In the autumn-winter period, they had access to Mombasa grass pasture and received roughage supplementations (black oat pasture; sorghum silage or sugarcane + urea) and pasture every 28 days, alternately. The animals had access to mineral mix and water and received 500 g of concentrated in each of the two milking times. The experimental design used was for response to continuous flow in rotational trial, similar to the Latin square model. In the spring-summer period, the average milk production was 12.2±2.2 and 14.0±3.8 kg/cow per day; for the autumn-winter period, the milk production was 13.5±2.9 and 12.1±2.5 kg/cow per day in the black oat pasture; 11.8±2.5 and 10.9±2.3 kg/cow per day in the sorghum silage and 11.3±1.8 and 8.4±1.5 kg/cow per day in the sugarcane + urea, for 1st and 2nd years evaluated, respectively. The black oat was the roughage supplementation that yielded the highest average daily milk production. There were no differences in the milk chemical composition between roughage supplementations.

Objetivou-se com a realização deste trabalho desenvolver modelos estatísticos para estimar as variações da população de protozoários ruminais em função da relação volumoso:concentrado e da presença ou não de aditivos na dieta. Foram construídas planilhas mediante a utilização do software Excel com dados referentes à composição da dieta, presença e ausência de aditivos e do número de protozoários do rúmen de bovinos de 23 artigos científicos e três teses realizados no Brasil. Estas variáveis foram utilizadas e testadas para obtenção da equação para predição do perfil da população de protozoários. Os modelos estatísticos obtidos foram verificados por análise residual e a seleção das variáveis foi realizada considerando o nível de significância de 5% de probabilidade do erro experimental. Verificou-se que dietas com maior proporção de volumosos favorecem o incremento na população de protozoários totais e em particular, de Entodinium, enquanto a presença de ionóforos reduz o número desses microorganismos ruminais. O modelo estatísticos desenvolvido pode auxiliar na identificação antecipada de fatores nutricionais limitantes para o desenvolvimento da microbiota ruminal.

The objective was to develop statistical models to estimate variations in the population of Protozoa in the light of forage: concentrate ratio and the presence or absence of additives in the diet. Excel spreadsheets with data on diet composition, presence and absence of additives and the number of protozoa in the rumen of cattle for work done in Brazil were using. These variables were used and tested to obtain the equation for predicting the profile of the population of protozoa. The models obtained were checked by residual analysis and selection of variables was performed considering the significance level of 5%. It was found for the total count of protozoa and Entodinium that the increased proportion of large positive effect and additive ionophores had a negative effect. The use of statistical models can provide a better understanding of dietary factors on the microbiota of the rumen and on the animal.

Objetivouse desenvolver modelos estatísticos que possam estimar as variações da população de protozoários ruminais mediante alterações na dieta, por meio de utilização dos parâmetros: teores de fibra em detergente neutro e proteína bruta, consumo de matéria seca e pH, bem como verificar se as variáveis peso vivo e raça exercem influência sobre a população de protozoários do rúmen. Foram construídas planilhas por meio do software Excel e registrados dados referentes aos parâmetros acima mencionados e sobre o perfil da microbiota ruminal. Estas variáveis foram utilizadas e testadas para obtenção das equações para predição do perfil da microbiota. Os modelos estatísticos obtidos foram verificados por análise residual e para a seleção das variáveis foi considerado o nível de significância de 5%, mediante a utlização do software MINITAB Versão 15 (2010). As equações de regressão obtidas a partir dos dados analisados permitem inferir que: ocorreu um aumento no número de protozoários totais proporcional à elevação do pH; as variáveis raça, peso e aditivos exerceram efeito significativo sobre a população de protozoários totais; à medida que elevouse o consumo de matéria seca, diminuiu o número de protozoários ruminais; o aumento do teor de fibra em detergente neutro na dieta promoveu incremento no número de protozoários; quanto maior o teor de proteína bruta, menor o número de protozoários totais. Os modelos obtidos permitem estimar o número de protozoários em função das variáveis raça, peso, pH, consumo de matéria seca, teores fibra em detergente neutro e proteína bruta da dieta e presença ou ausência de aditivos.

The objective was to develop statistical models that can estimate the variations of the rumen protozoa population through changes in diet, using as parameters: neutral detergent fiber and crude protein, dry matter intake and pH, and to verify whether the variables weight and race influence on the population of protozoa in the rumen. Were built using Excel spreadsheets and recorded data on the aforementioned parameters and the profile of the rumen microbiota. These variables were used and tested to obtain the equations for predicting the profile of the microbiota. The models obtained were checked by residual analysis and selection of variables was performed considering the significance level of 5%, using the software MINITAB Release 15 (2010). The regression equations obtained from the analyzed data may imply that: there was an increase in the number of total protozoa proportional to the elevation of pH, the variables race, weight and additives exerted a significant effect on the protozoa population totals, as increased if dry matter intake decreased the number Protozoa; increasing the neutral detergent fiber content in the diet promoted an increase in the number of protozoa, the higher the crude protein, the lower the total number of protozoa. The models allow to estimate the number of parasites in the light of these variables: pH, dry matter intake, neutral detergent fiber and crude protein contents of diet and the presence or absence of additives.

The Cornel Net Carbohydrate and Protein System model version 5.0 was assessed as for its prediction of dry matter intake and milk production of grazing lactating Holstein × Zebu cows. Eight lactating cows grazing elephant grass (Pennisetum purpureum, Schum., cv. Napier) were used in two experiments of 30 days each. The experimental design was of randomized blocks (subplots). Dry matter intake was estimated using the chromium oxide and in vitro dry matter digestibility techniques. The estimated dry matter intake was compared with the values predicted by the model. Extrusa samples of the elephant grass pasture were obtained from a cow with esophageal fistula during nine days in each experimental period. Carbohydrate and nitrogenous fractions were analyzed and the degradation rate of carbohydrate was estimated by the gas production technique. The inputs referring to the animals (body weight, age, milk production, milk composition and breed type), to the environment (temperature, relative humidity of air and management condition) and to feeds chemical composition in each experimental period were inserted in the model. The model was accurate on the prediction of dry matter intake, considering that the mean values obtained by the chromium oxide technique and predicted by model (2.45 and 2.46% of body weight, respectively) did not differ significantly from each other. However, the model underestimated the observed milk production in 62.4%, where the metabolizable energy was the first limiting. These results demonstrate the need for studies that provide the development of a database for tropical foods (chemical composition and dynamics of rumen fermentation) and fitting of the model to the tropical conditions, for its appropriate application in formulation of diets and supplements in these regions.